FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l07n01 2-3h db 8wg16 rep1 input_150303.34100000007c28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l07n01 2-3h db 8wg16 rep1 input_150303.34100000007c28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51051849
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT2877810.5637033832016545No Hit
AAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA2753930.539437856599474No Hit
AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA2742040.537108851826307No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA2440050.477955264656526No Hit
TCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT2306640.45182300840856915No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG2063190.40413619494956976No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTC1809150.35437501979605085No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG1792880.35118806372713357No Hit
AAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACA1699120.3328224213779211No Hit
CAACACAACACAACACAACACAACACAACACAACACAACACAACACAACAC1670510.32721831485476655No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC1619250.31717754238441No Hit
CAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGAC1610980.31555762064563025No Hit
AACACAACACAACACAACACAACACAACACAACACAACACAACACAACACA1546600.3029469118738481No Hit
CACAACACAACACAACACAACACAACACAACACAACACAACACAACACAAC1478480.2896036145527266No Hit
AGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAA1344820.26342238848195293No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT1338530.2621903077398823No Hit
ACAACACAACACAACACAACACAACACAACACAACACAACACAACACAACA1257400.24629862083937448No Hit
ACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGA1197690.2346026683578101No Hit
TGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGT1125780.22051698852278592No Hit
GTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTG1088830.21327924871046297No Hit
ACACAACACAACACAACACAACACAACACAACACAACACAACACAACACAA1025700.20091338905276473No Hit
TGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTT1022550.20029636928527308No Hit
GTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTG950190.18612254376917867No Hit
GACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAG925960.18137638854177446No Hit
TTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGT766730.15018652899329857No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC698850.13689024270208117TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA146800.023.5979416
CACGTCT150650.023.09939614
ACGTCTG149550.023.07376115
CTCGTAT150600.022.73573942
ACACGTC153300.022.6120513
AGTCACC152050.022.47123128
TCGTATG156200.022.0813343
GTATGCC163950.021.10641945
TCTGAAC165900.021.08450518
TCTCGTA166050.020.64740841
CAGTCAC175000.019.75677127
CGTATGC175800.019.73490744
ATCTCGT175450.019.47751840
GTCACCA178650.019.3789129
CCAGTCA181250.019.1874826
CACACGT183250.019.1373412
ACCAGAT184750.018.743932
TCCAGTC186800.018.64150425
AGATCAT185900.018.52973735
GTCTGAA191150.018.40525617
Produced by FastQC (version 0.11.2)