FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l07n01 2-3h db 8wg16 rep1_150303.34100000007be3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l07n01 2-3h db 8wg16 rep1_150303.34100000007be3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46450288
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC14456093.1121636963800956TruSeq Adapter, Index 3 (100% over 51bp)
AAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA1707730.36764680554833157No Hit
AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA1565240.3369710000506348No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA1356460.2920240236185403No Hit
TTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT1199590.2582524353777957No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG1139990.2454215138558452No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC1129700.24320624233804536No Hit
TCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT1108200.2385776380977444No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG1054210.2269544593566352No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT1004900.21633880935248453No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTC988860.2128856553053019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT1569900.043.2699214
ACACGTC1574900.043.2325313
CGTCTGA1571250.043.23130816
ACGTCTG1574550.043.1578515
CTTAGGC1558100.043.0677333
ACTTAGG1567050.042.9788532
TCGGAAG1594600.042.8860473
TCTCGTA1566450.042.8346641
CACACGT1594250.042.72896612
TAGGCAT1573150.042.6686335
CGGAAGA1602050.042.6332554
GTATGCC1576300.042.6307445
TCGTATG1579100.042.5575443
GATCGGA1611950.042.5426061
CACTTAG1585500.042.4718831
ATCTCGT1580600.042.4518340
CTCGTAT1582300.042.40273742
CGTATGC1583600.042.36744744
TCTGAAC1604000.042.33039518
AGGCATC1585950.042.25361636