FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l06n01 k38.34100000007f25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l06n01 k38.34100000007f25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17946094
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG920510.5129305574795273No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC908200.5060711261180288TruSeq Adapter, Index 8 (100% over 51bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG697380.3885970952787832No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG624790.34814818199436603No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA449390.25041103651858726No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC403070.2246004060827944No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC383370.21362308700712254No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT351180.19568603619261105No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT341700.19040354965264308No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA316040.17610517363834158No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG283470.1579563775827765No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC280600.1563571437885035No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT262160.14608192735421982No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT257820.14366357381166064No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC247030.1376511234143764No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT242090.13489843528068002No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT237060.13209559695831305No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG232810.12972739360442445No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT230940.12868538412871347No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA217960.12145261247377842No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA188420.10499220610345628No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC185340.10327595520228525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG108500.039.91284611
CGTATGC106850.039.77522744
ACACGTC111950.038.6828413
CACACGT112500.038.4737312
GTATGCC111100.038.3541845
ACGTCTG113600.038.06157315
TCGTATG111850.037.99695243
CTCGTAT114000.037.3390342
AGCACAC117250.037.06857710
CACGTCT117650.036.73221614
GTCACAC118300.036.05716329
CGTCTGA121500.035.67767316
TCTCGTA121250.035.16205241
CAGTCAC124000.034.56374727
GAGCACA130600.033.710059
CTGAACT130950.033.2405219
CACACTT129250.033.18843531
AGAGCAC132900.033.0065968
TCGGAAG131750.032.9019933
ACTCCAG132800.032.8874323
Produced by FastQC (version 0.11.2)