FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l06n01 k37.34100000007f18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l06n01 k37.34100000007f18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16504064
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC823710.49909525314492237TruSeq Adapter, Index 7 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG718710.43547455947819885No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG559750.33915888838046193No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG457770.277368047045867No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA344890.20897277179729792No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC316230.19160735198312367No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC303090.1836456766042594No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT275310.16681345879414913No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT245640.14883606849803782No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG243810.14772725069413206No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA242980.14722434425848083No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC220850.13381552567900853No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT214140.12974986039802075No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG191040.11575330779134158No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC190740.11557153438086522No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT189110.11458389885061038No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT188630.1142930613938482No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT182570.11062123850222587No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT179490.10875503148800199No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA171750.10406527749771209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG101250.040.4160211
CGTATGC100150.039.71901744
CACACGT105050.038.88979312
GTATGCC103100.038.6028445
ACACGTC106200.038.4264213
ACGTCTG106650.038.26428615
TCGTATG106250.037.50208743
CACGTCT110550.036.8940414
AGCACAC111050.036.86961710
CTCGTAT108950.036.6343342
CATCTCG110550.036.38904639
CGTCTGA115550.035.3341716
GAGCACA116850.034.9240349
TCTCGTA115300.034.7728541
CAGTCAC116200.034.63533427
AGTCACC117250.034.20915628
AGAGCAC122350.033.44388
CTGAACT122900.033.2760319
TCGGAAG126500.032.27553
CCAGTCA125850.032.08686426
Produced by FastQC (version 0.11.2)