FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l06n01 k35.34100000007ee0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l06n01 k35.34100000007ee0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19626989
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC2259391.1511648577374756TruSeq Adapter, Index 5 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG811260.413338999680491No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG663610.33811095527694035No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG550310.28038432181319306No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA455180.23191534880872455No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC379480.19334600941591196No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC374750.1909360625819885No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT327770.1669996350433579No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA312290.15911253631415395No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT298690.15218330228849672No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG293200.14938613355313948No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG279670.14249256470261434No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT272600.13889038201427636No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA252320.12855767127601692No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC252120.1284557707756396No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT238080.12130235564915229No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC236280.12038525114575649No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT212900.1084730826516487No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT211960.10799415029987534No Hit
CCGGCGCCTACAAGACCGACATCAAGCTGGACATCACCTCCCACAACGAGG198500.10113624662448223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG255600.042.22022611
CGTATGC253050.041.8235944
GTATGCC253900.041.69148345
ACGTCTG259800.041.4165615
CACACGT261000.041.31222512
TCGTATG257400.041.11646743
ACACGTC262300.041.07327713
AGCACAC266050.040.62107510
CACGTCT264850.040.57588614
CTCGTAT261150.040.4824642
CACAGTG263900.039.98133533
GTCACAC265100.039.88857329
CGTCTGA270550.039.71961216
TCTCGTA267800.039.51910841
CACACAG269300.039.37936831
ACACAGT269600.039.1553232
CAGTCAC269900.039.0881427
CTGAACT274950.039.02671419
GAGCACA278650.038.8488549
TCGGAAG280900.038.5202873
Produced by FastQC (version 0.11.2)