FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l06n01 k33.34100000007ec6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l06n01 k33.34100000007ec6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18206416
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC1546460.8494038585079019TruSeq Adapter, Index 3 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG771430.4237132667956176No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG624040.34275828916575346No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG503440.27651790445741764No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA419120.23020456085371221No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC361120.1983476594185259No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC325880.17899184551204367No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT304040.1669960743509321No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT299210.16434316342107091No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA278050.15272088696644084No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG258630.14205431755486636No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT243690.13384841915069942No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC237230.1303002194391252No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG233990.12852062701412512No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA226540.12442866295046756No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT219380.12049598339398594No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC218850.12020487722569889No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT198300.10891764749305959No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT193560.10631416968611504No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA185690.10199151771551303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG181350.041.85436611
CGTATGC180600.041.49699844
GTATGCC181300.041.36057345
ACGTCTG184950.041.00353215
ACACGTC185600.040.8599313
ACTTAGG184100.040.7916132
TAGGCAT183400.040.63839735
CACTTAG184950.040.6062531
CACACGT187150.040.4974812
TCGTATG186300.040.22702843
CTCGTAT187650.039.8892342
CACGTCT190700.039.77898414
AGCACAC191500.039.74170310
TTAGGCA188500.039.53889534
CATCTCG191000.039.15426639
TCACTTA191400.039.1504630
CGTCTGA194950.038.94373316
AGTCACT193700.038.70882428
TCTCGTA195750.038.3420841
CAGTCAC196150.038.3187227
Produced by FastQC (version 0.11.2)