FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l06n01 k312.34100000007f75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l06n01 k312.34100000007f75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17213728
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC1052980.6117094449267468TruSeq Adapter, Index 12 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG851860.4948724645817571No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG574390.33368135014100375No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG557170.32367770653747985No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA382710.22232836489573904No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC377060.21904610087948412No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG283440.164659276595982No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC273300.1587686293172519No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA269720.1566888938874833No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT267490.15539341623151012No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT259580.1507982466087532No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT241060.14003939181564853No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC235390.13674550916570774No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT226280.13145322152179933No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG207640.12062465492657953No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT203760.11837063999152303No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA197020.11445516043938884No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA194390.11292731010969848No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG186870.10855870384381582No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC183250.1064557311466755No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT177830.10330708141780792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC122850.040.72999244
GCACACG128650.040.02582611
ACGTCTG129950.039.57347515
GTATGCC126550.039.46689245
CACACGT130950.039.42588412
TCGTATG128750.038.88078343
ACACGTC132850.038.7604213
CTCGTAT132050.037.8233942
AGCACAC137250.037.68173610
CACGTCT136650.037.64963514
CGTCTGA141050.036.60159316
AGTCACC139850.035.92555628
CAGTCAC140000.035.88883627
TCTCGTA140700.035.60969541
GAGCACA146700.035.43849
CTGAACT148200.034.80557319
TGTAATC144600.034.59947236
AGAGCAC152100.034.105198
CCAGTCA148500.034.01650226
TCGGAAG153300.033.7795263
Produced by FastQC (version 0.11.2)