FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l06n01 k311.34100000007f5c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l06n01 k311.34100000007f5c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15894823
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC1511850.9511587515004098TruSeq Adapter, Index 11 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG654360.41168121217833004No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG479700.30179637734877574No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG433030.272434615975277No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA361210.2272500926874115No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC295640.1859976672907902No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC284660.17908975771545238No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT240560.15134487499483323No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT228810.1439525309592941No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG224850.14146115373540177No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA222720.14012109477406573No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT218510.13747243363452366No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG190700.1199761708576434No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC177890.11191694301974926No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT171360.1078086871429773No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT163890.10310904374336222No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT163060.102586861143405No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA162350.10214017482295965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG176550.042.10111611
CGTATGC171400.041.3086844
ACACGTC180750.041.07304413
GTATGCC172600.041.00677545
ACGTCTG181900.040.78864315
CACACGT182600.040.64459612
AGTCACG178600.040.31543728
CACGGCT178950.040.23151831
ACGGCTA179550.040.2086332
AGCACAC185550.040.11964810
TCGTATG177000.040.0778843
CGGCTAC179300.039.97421633
CACGTCT185850.039.93383814
CTCGTAT177500.039.85053642
GGCTACA180400.039.4561234
CGTCTGA189950.039.0933516
CATCTCG181300.039.06503739
GCTACAT184100.038.67535835
TCTCGTA183950.038.53895641
GAGCACA193950.038.4400529
Produced by FastQC (version 0.11.2)