FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l06n01 k310.34100000007f4f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l06n01 k310.34100000007f4f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16755656
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC1214950.7250984384019342TruSeq Adapter, Index 10 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG794280.4740369460915168No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG568260.3391451817821994No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG536920.3204410498759344No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA436110.260276291181915No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC350070.20892646638245616No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT312050.18623562097479204No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC297530.17756989042983456No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA290660.17346978238273691No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT286030.17070653634808447No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG272480.16261971479958767No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT263300.1571409678021559No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG238850.14254888021095682No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC217840.13000983071029867No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA217600.12986659549467952No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT214210.12784339807405928No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT203830.12164847499853183No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT193560.11551920139682983No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC188570.1125411025387487No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT184270.1099748049255726No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA183220.1093481508572389No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGC172480.10293837495828274No Hit
CCGGCGCCTACAAGACCGACATCAAGCTGGACATCACCTCCCACAACGAGG170380.10168506682161534No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG167810.1001512563876938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC135150.040.85223444
GCACACG138600.040.7561211
GTATGCC136450.040.49539645
ACGTCTG139600.040.3838415
ACACGTC139700.040.3066213
CACACGT140700.040.13183212
CACGTCT142900.039.40402614
TCGTATG141650.039.16798443
TATCTCG141950.038.97402639
AGCACAC146000.038.81366710
TAGCTTA144100.038.6703334
CTCGTAT143400.038.5328742
CACTAGC146850.037.96236431
GTCACTA146500.037.9201229
CGTCTGA148800.037.90029516
TCACTAG147300.037.6988930
AGTCACT149950.037.21301328
ACTAGCT150600.037.09313232
TCTCGTA149250.037.02253341
CAGTCAC151750.036.63897727