FastQCFastQC Report
Mon 13 Apr 2015
C55MVACXX l05n01 k19.34100000008348.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l05n01 k19.34100000008348.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14533319
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC857290.5898790221283934TruSeq Adapter, Index 9 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG429610.2956035025447387No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG305030.20988323451786892No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG285090.1961630375002434No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA226600.15591758496459066No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC206510.1420941768360001No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC170100.11704139983440809No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA149480.1028533124470742No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT148720.10233037615151777No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT145390.10003908948809284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG103500.040.14311211
GTATGCC102450.039.9942545
CGTATGC102500.039.95292344
AGTCACG105250.039.008528
GTCACGA104850.039.00556629
ACGTCTG107050.038.81201615
AGCACAC109250.038.03031510
CTCGTAT107950.038.02079442
CACACGT110800.037.6607212
TCGTATG109800.037.4410243
ACACGTC111100.037.35655613
CGTCTGA112700.036.90617416
CACGTCT112600.036.83905814
AGAGCAC117550.035.4216088
GAGCACA118050.035.1953629
CAGTCAC117050.035.0582127
TCTCGTA117350.034.97355741
TCACGAT117650.034.8563230
CGATCAG119650.034.29300733
CTGAACT122450.033.875819