FastQCFastQC Report
Mon 13 Apr 2015
C55MVACXX l05n01 k17.3410000000832e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l05n01 k17.3410000000832e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18395331
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1424720.7745008774237333TruSeq Adapter, Index 7 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG896650.4874334688514167No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG759920.41310482534943244No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC430710.23414093500138702No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG427360.232319820719725No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT360020.1957127055773011No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT294570.16013302505945665No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA284170.1544794165432522No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA276080.1500815614570893No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC259470.1410520963172666No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC258150.1403345229286714No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT250370.13610518886558767No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT247650.13462655279211883No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG247120.1343384362042738No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT231570.12588520424014116No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC230790.1254611836014258No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA229490.12475448253690027No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG225520.1225963262090799No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT223980.12175915725571886No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT212260.11538797535091921No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG211110.11476281671691582No Hit
GGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATC185520.10085167806983197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG165650.041.67775711
GTATGCC163900.041.55443245
CGTATGC165550.041.1403844
ACGTCTG169250.040.724815
AGCACAC171650.040.26023510
CTCGTAT170500.040.0139242
ACACGTC172850.039.85058213
CACACGT174450.039.52377312
CACGTCT175900.039.21075414
TCGTATG174350.039.1936943
CGTCTGA177250.038.95018816
CATCTCG176650.038.65654439
GAGCACA180350.038.517679
AGAGCAC181300.038.2413948
CAGTCAC179750.038.08049827
TCTCGTA179600.037.97148541
AGTCACC180450.037.7074328
CTGAACT184100.037.4890119
TCGGAAG186900.037.011433
CCAGTCA188550.036.30320726
Produced by FastQC (version 0.11.2)