FastQCFastQC Report
Mon 13 Apr 2015
C55MVACXX l05n01 k15.34100000008304.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l05n01 k15.34100000008304.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24554146
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1539010.6267821328422499TruSeq Adapter, Index 5 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG1041270.4240709491586472No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG850170.34624295220855983No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG474860.1933930017358372No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC472430.19240335216708412No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT413860.16854994671775592No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA402750.164025252598889No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT347640.14158097781124213No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA318030.12952191454754727No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG305350.12435781720936254No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC297230.1210508400495786No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT295860.1204928894696643No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT293750.1196335641239569No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC287830.11722256599761198No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG285860.11642025749948705No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC284230.11575641848834817No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT263910.10748083032494798No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT257180.10473994900901869No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA248270.10111123392359074No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT247120.10064288124702037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG181850.040.64831511
GTATGCC180150.040.46697645
CGTATGC180250.040.45700544
ACGTCTG185100.039.77670315
AGCACAC190350.038.90408710
ACACGTC192150.038.42257713
CTCGTAT189300.038.4179142
CACACGT192650.038.25279612
CACGTCT193900.037.98306714
TCGTATG192200.037.91872843
CGTCTGA197600.037.3627716
CACAGTG194650.037.29461333
GAGCACA201100.036.9586649
ACACAGT196750.036.90956532
AGAGCAC201500.036.8516548
CACACAG200350.036.4985831
GTCACAC201550.036.19437429
CTGAACT207200.035.78425219
TCTCGTA203800.035.6606541
CAGTCAC205450.035.60859327