FastQCFastQC Report
Mon 13 Apr 2015
C55MVACXX l05n01 k14.341000000082f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l05n01 k14.341000000082f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19516734
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC1214460.622266000038736TruSeq Adapter, Index 4 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG738920.3786084290537546No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG618960.31714322693540836No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG354060.18141355003352508No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC328890.16851692501419552No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA314900.16134871746471516No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT289390.14827788296955832No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT224560.11506023497579053No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA223210.11436852088059406No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC220810.11313880693357814No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT219400.11241634998970626No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT216050.11069987427199653No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC215880.11061276953408292No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC213310.10929595084915335No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT196290.10057522944156537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG146100.041.09457811
CGTATGC146300.040.21912444
GTATGCC146450.040.11606245
ACGTCTG151450.039.5687415
AGCACAC153950.039.08681510
ACACGTC154900.038.65830613
CTCGTAT153650.038.2235542
CACACGT156550.038.2077512
ACTGACC154650.038.0837132
TCGTATG155500.037.8984543
CACGTCT158950.037.7583114
CGTCTGA161850.037.19295516
AGTCACT162050.036.58624328
AGAGCAC165800.036.4695938
GAGCACA166000.036.4256559
CACTGAC163600.036.111931
CAGTCAC164750.036.03013227
CTGAACT168900.035.6407719
TCTCGTA164850.035.59694741
TCGGAAG174900.034.6236233
Produced by FastQC (version 0.11.2)