FastQCFastQC Report
Mon 13 Apr 2015
C55MVACXX l05n01 k13.341000000082e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l05n01 k13.341000000082e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17796522
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC1391850.7820910175594985TruSeq Adapter, Index 3 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG717040.40291018660837213No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG612410.3441178000959963No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG334980.18822778967710657No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC329850.18534520396738194No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA289510.1626778535716136No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT288720.16223394661046692No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT244100.13716163191886593No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA229830.12914321124093797No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC218400.12272060799295502No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT215010.1208157414128446No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT211070.11860182568256877No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC207060.11634857642409005No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG206510.11603952727392464No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG200680.11276360628217132No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT193290.10861110951903973No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC191930.10784691525681253No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA178400.10024430616274349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG161800.041.7787911
GTATGCC160150.041.77030645
CGTATGC161350.041.5154344
TAGGCAT163350.040.79317535
ACGTCTG166450.040.59835415
CACTTAG166050.040.3621831
ACTTAGG166400.040.2075932
AGCACAC168250.040.20390310
CACACGT168700.040.0299912
CTCGTAT169000.039.59846542
TCGTATG169850.039.55760643
ACACGTC171800.039.25530213
TTAGGCA169650.039.2239434
AGGCATC170050.039.138536
CACGTCT174850.038.58363314
CATCTCG173900.038.44236439
GGCATCT174450.038.16584437
CGTCTGA177200.038.10981416
TCACTTA175800.038.0225330
AGAGCAC178850.037.9089438
Produced by FastQC (version 0.11.2)