FastQCFastQC Report
Mon 13 Apr 2015
C55MVACXX l05n01 k12.341000000082dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l05n01 k12.341000000082dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16126887
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC926100.5742583798100651TruSeq Adapter, Index 2 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG725950.4501488725009359No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG636650.39477550751115204No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC332030.20588598407119738No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG321940.19962935190157902No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT300650.18642779601543683No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA287030.1779822727101641No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA245920.15249068217567346No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC240530.14914843763709637No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT235380.14595501289244478No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC228500.14168884546658014No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT225100.13958056505263539No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT218100.135239987729808No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG212580.13181713246952126No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT210200.13034133617975993No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG209530.12992588092171786No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC187710.11639568132399017No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA181670.11265038317686482No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT181530.11256357163040827No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG179780.11147842729970142No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT173390.1075161002864347No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA168380.10440948708823966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG113150.040.716411
GTATGCC112250.039.98914345
CGTATGC113000.039.84356744
ACGTCTG117700.039.00861415
AGCACAC118600.038.9591710
ACACGTC119500.038.4963313
CACACGT119900.038.42419412
CTCGTAT118000.038.2717142
CACGTCT121600.037.73901714
TATCTCG120800.037.5315439
TCGTATG120400.037.4890843
CGTCTGA123850.037.14422216
GAGCACA126900.036.3932889
CGATGTA125100.036.19810534
AGAGCAC128650.035.968188
AGTCACC127050.035.85741428
CAGTCAC127400.035.67211527
CTGAACT129600.035.54852719
TCTCGTA128000.035.33249741
CCGATGT129800.034.8178533
Produced by FastQC (version 0.11.2)