FastQCFastQC Report
Mon 13 Apr 2015
C55MVACXX l05n01 k112.3410000000837e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l05n01 k112.3410000000837e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13685390
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC738460.5395973370141443TruSeq Adapter, Index 12 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG485840.3550063242625895No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG388030.28353594599788534No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG285110.20833165879817822No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC230530.16844971169984926No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA195600.14292614240441814No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC180770.13208976872416495No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT171140.12505306754137074No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA155460.11359559354903294No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT151660.11081890980088986No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT142120.10384797218055168No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG139480.10191890768184172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG89650.040.0727711
GTATGCC88050.039.91681345
CGTATGC88950.039.53837244
ACGTCTG92950.038.50499315
CTCGTAT92750.037.87119742
ACACGTC94400.037.81809213
AGCACAC95100.037.79992310
CACACGT95200.037.7129612
TCGTATG94100.037.39907543
CACGTCT98150.036.37331414
CGTCTGA99150.036.09708416
GAGCACA102050.035.269699
AGAGCAC102300.035.161388
CAGTCAC100950.035.1175727
CTGAACT103550.034.71584319
TCTCGTA101800.034.63505641
AGTCACC104400.033.89078528
CCAGTCA106200.033.4239226
TCGGAAG108400.033.1204873
TCCAGTC108600.032.83047525
Produced by FastQC (version 0.11.2)