FastQCFastQC Report
Mon 13 Apr 2015
C55MVACXX l05n01 k111.34100000008361.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l05n01 k111.34100000008361.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13059003
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC720390.5516424186440573TruSeq Adapter, Index 11 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG399130.30563588966171457No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG288840.22118074404301769No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG267000.20445664956199183No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA196170.15021820578492862No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC179260.13726928464600244No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC164060.12562980497056322No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT145000.11103451006175585No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT138870.10634043042948992No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA138100.10575079889329989No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG137590.10536026371997924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG87700.040.4242211
GTATGCC85800.039.73166745
CGTATGC86800.039.32607744
ACGTCTG91750.038.54190415
AGCACAC92650.038.2887610
AGTCACG91300.038.06341628
CACACGT93300.037.9737912
CGGCTAC91050.037.72658533
ACACGTC93700.037.71565213
ACGGCTA91850.037.62057532
CACGGCT92000.037.54973231
CTCGTAT90900.037.48047342
TCGTATG92400.036.99265743
GGCTACA92700.036.9344534
CACGTCT95850.036.79939314
CGTCTGA98250.036.10640316
GCTACAT95200.035.96494735
GAGCACA100250.035.520719
AGAGCAC100700.035.451198
CATCTCG96550.035.40084539