FastQCFastQC Report
Mon 13 Apr 2015
C55MVACXX l05n01 k110.34100000008355.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l05n01 k110.34100000008355.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15737817
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC935270.5942819134318311TruSeq Adapter, Index 10 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG557780.354420184197084No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG374530.23798090929637827No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG346900.22042447183113134No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA273030.1734865769502848No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC232910.147993841839691No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC214570.1363403831675003No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT179850.11427887362014694No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT178420.11337023425802956No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA177040.11249336550297921No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG165900.10541487424844247No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT165440.1051225846634257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG106350.040.44262711
GTATGCC104400.039.6776345
CGTATGC105250.039.33606344
ACGTCTG113450.038.0506615
AGCACAC113250.037.93884710
ACACGTC115100.037.46586213
CTCGTAT110700.037.46175442
CACACGT116500.037.0156312
CACTAGC112100.036.96209731
TCGTATG112550.036.94548443
TAGCTTA113550.036.55299834
TATCTCG114300.036.4568339
CGTCTGA119150.036.24889416
CACGTCT120250.035.9550614
ACTAGCT115200.035.9135432
GTCACTA116650.035.5040129
GAGCACA121950.035.4536139
AGAGCAC123250.035.0428248
AGTCACT118750.034.95285428
CAGTCAC120550.034.6189927
Produced by FastQC (version 0.11.2)