FastQCFastQC Report
Mon 13 Apr 2015
C55MVACXX l05n01 k11.341000000082cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l05n01 k11.341000000082cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22020475
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG857200.3892740733340221No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG750570.3408509580288345No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG412820.18747097871412854No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC410160.1862630120376604No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT355240.16132258727388943No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA333510.15145449859732815No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT284480.1291888571885938No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC273860.12436607293893524No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA264860.12027896764261443No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT264240.119997411499979No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT252700.11475683426447432No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG250430.11372597548418006No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC249450.1132809351296918No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG247330.1123181947710029No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT236280.10730013771274236No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC234650.10655991753129757No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA221100.10040655344628124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT82500.023.4418871
GCACACG26550.021.85972811
CGTATGC27100.020.5081644
GTATGCC26800.020.48566845
CTGACTC102100.019.102182
ACTCTAG110300.018.0695695
GACTCTA115950.017.4024854
ACGTCTG33550.017.1647715
CTAGCAG113750.017.0864528
CTCGTAT34600.016.25909842
TGACTCT125700.015.9989623
AGCACAC38850.015.51791410
CACACGT37850.015.45243912
ACACGTC38200.015.19308413
TCGTATG37500.014.88100543
CTCTAGC134550.014.8463216
TAGCAGA135550.014.4712679
AGAGCAC43450.013.8232788
CGTCTGA43600.013.5177816
CACGTCT42800.013.45509414
Produced by FastQC (version 0.11.2)