FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l04n01 es-nil 2nd.34100000007c35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l04n01 es-nil 2nd.34100000007c35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69030049
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGA84250.020.06459830
GATCGGA81950.019.7805521
ACACGTC95400.018.08654813
TATCTCG94650.017.8507339
ATCGGAA91550.017.6431482
ACGTCTG100600.017.17399215
TCGTATG105200.016.93730443
CGTCTGA106400.016.36461616
CACCGAT104750.016.3091931
GTCACCG108250.015.88590729
CGTATGC118100.014.57300844
CGATGTA126050.013.8156734
TCGAATG117000.013.6323382
CACACGT131100.013.4359312
CTCGTAT130050.013.18195242
ACCGATG133450.012.88144732
CACGTCT138950.012.87112914
TCTCGTA139550.012.4135541
GTATGCC142900.012.1853145
CGAATGT110100.011.9326113
Produced by FastQC (version 0.11.2)