FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l04n01 eb-nil-48hr dox 2nd.34100000007d0d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l04n01 eb-nil-48hr dox 2nd.34100000007d0d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32652859
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG29450.015.20224141
CCGTATC29200.015.02377238
TCCGTAT33050.014.0219937
TCGTATG39000.012.1138645
GATCGGA36750.011.7788221
GTTCCGT40650.011.29114735
TCGAATG45250.011.1858892
ATCGGAA38800.011.1320742
AGTTCCG40900.011.05709934
ACACGTC43950.010.80012713
CGTCTGA45200.010.45164816
CTCGAAT86500.010.1389141
ACGTCTG46400.010.13288115
TCTCGTA52850.09.74833243
AATCCGA49000.09.5067624
CGAATGT47300.09.3218373
CGCTAAT8100.09.18516351
CGTATCT49750.09.180016539
CTCGTTT59650.08.9954821
CTCGTAT53000.08.9567544
Produced by FastQC (version 0.11.2)