FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l04n01 eb-nil-36hr dox 2nd.34100000007cd5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l04n01 eb-nil-36hr dox 2nd.34100000007cd5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37457089
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATG56700.012.7756532
ATCGGAA41800.010.9790352
CGGCTAT30400.010.9749651
AATCCGA70100.010.85077924
CGCGTAA6250.010.8207231
ACACGTC45300.010.67703213
CTCGAAT118200.010.661281
ACGTCTG46250.010.45767815
TCGTATG50000.010.303699545
CGTCTGA47800.010.11855616
CTCGTTT78000.010.0577231
GATCGGA46900.09.9497591
AAATCCG74700.09.93912723
ATCCGAA76700.09.91876925
CGCTAGT8000.09.5808491
TATCATG79500.09.3946146
CGATTGG33300.09.390334
CGGGAAT32450.09.3785161
AATCTCG50750.09.35359941
CGAATGT59900.09.3515343
Produced by FastQC (version 0.11.2)