FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l04n01 eb-nil-24hr dox 2nd.34100000007caf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l04n01 eb-nil-24hr dox 2nd.34100000007caf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29600125
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAC10900.010.94511829
TCGAATG38150.010.614162
AATCCGA45500.010.53490724
GACGCGA11850.010.06706728
CGATTAG29850.09.872674
CGGCTAT18600.09.8176111
ATCCGAA49850.09.61751425
CGAATCG7750.09.5789943
CGGCATT26250.09.5329511
AAATCCG47050.09.42035223
CTCGAAT83200.09.4024251
TTACGGC20750.09.21576140
CCGCTAT13550.09.150761
CGGGTAT16950.09.0442581
ATTACGG30500.08.9989239
GTCGGTT40800.08.730331
CGCGACT13450.08.70300230
CGCATAT12650.08.5542991
CTCCGAT58800.08.5499051
GATTACG17750.08.49166637
Produced by FastQC (version 0.11.2)