FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l04n01 eb-nil-12hr dox 2nd.34100000007c85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l04n01 eb-nil-12hr dox 2nd.34100000007c85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39784327
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA51300.014.9098062
ACACGTC57500.013.69339713
GATCGGA59200.013.0614711
TCGTATG65250.012.44675943
ACGTCTG64450.012.42613115
CGTCTGA67950.011.45496716
CACACGT80750.010.64221212
TCTCGTA78150.010.21948341
CGTATGC78550.010.19622544
CACGTCT79700.010.18961414
GATTACG23350.010.11629937
CGCTAAT7850.010.0512031
CTCGTAT79550.09.98306942
CGGCTAT31650.09.7581281
CCGATTT39100.09.68617251
TCGAATG50000.09.6284032
CCGGCAT54100.09.6257461
CCGGGAT57300.09.5209521
GCGGAAT31050.09.2206551
CGTACGA27000.09.08477224
Produced by FastQC (version 0.11.2)