FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l04n01 eb-nil-0hr 2nd.34100000007c6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l04n01 eb-nil-0hr 2nd.34100000007c6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28276904
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC329030.11635998056930136TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA59950.027.9183432
ACGTCTG64200.027.43655215
CGTCTGA64500.027.09967616
ACACGTC64400.027.0719913
AATCTCG68800.025.14681239
TCGTATG71650.024.74293543
GATCGGA68550.024.49981
CACGTCT76950.022.94900314
CACACGT78050.022.62564812
CGTATGC76600.022.26297244
CTCGTAT81850.021.38464742
TCTCGTA80650.021.36808441
GTATGCC90100.019.30130645
CGGAAGA91650.018.8264644
ACTGACC94000.018.52676432
ACCAATC96100.018.21466636
GACCAAT94350.018.12349935
GCACACG110700.016.23691211
TCGGAAG110600.015.6007723
GTACACT113700.015.564061
Produced by FastQC (version 0.11.2)