FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l03n01 es-nil.34100000007d84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l03n01 es-nil.34100000007d84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28221791
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATG46450.013.3664662
AATCCGA50150.013.05726524
ATCCGAA53400.012.30670525
AAATCCG54850.011.40281723
CGAATGT43600.011.1444973
CGGCTAT17000.010.4764141
CTCGAAT94700.010.4507881
GTCGCAT18800.010.1928471
CGCTAGT6250.010.09979251
CTCGTTT54850.09.9054651
GCGGCTT66100.09.69985115
GTCGGTT47450.09.6448241
TGCGATA33350.09.51100639
CTCGATT19050.09.4673731
TCGTTTT86650.09.3200682
CGATAAC25750.09.2803231
CGCATAT10950.09.2647321
TATCATG68150.09.24241356
GCGATAA36000.09.185822540
CGGGAAT24300.09.0918921