FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l03n01 eb-nil-48hr dox.34100000007e83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l03n01 eb-nil-48hr dox.34100000007e83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27248923
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG17100.013.02820732
AATCCGA46650.012.20396324
AAATCCG47050.011.47601723
ATCCGAA49600.011.34403825
TCGAATG39200.010.2149642
CGGCTAT18500.09.6261471
CGCATCG13250.09.3380338
CTCGAAT70050.09.23572351
TATCATG63650.09.0124546
CGTCCGC26900.08.95017733
GCGGCTT64250.08.71870915
CTCAGAT79850.08.6103751
CGAATGT38450.08.6005033
GTATCAT64800.08.5747855
GTCGGTT36450.08.5345121
CGTATCG8300.08.40245917
GTCGAAT34650.08.392351
CGATTGG21800.08.358564
ATCATGC82150.08.3246917
TCGTTTT61600.08.2533572
Produced by FastQC (version 0.11.2)