FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l03n01 eb-nil-36hr dox.34100000007e69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l03n01 eb-nil-36hr dox.34100000007e69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73533294
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGA135250.012.97748724
TCGAATG121200.012.0087522
ATCCGAA154300.011.47932925
AAATCCG151750.011.282392523
CTCGAAT222900.011.1254841
TATCATG162700.010.8260466
CGCATCG49500.010.49823438
GTATCAT166500.010.4978995
AATAACG52400.010.13650927
CGAATGT111900.010.0717253
TCGTTTT179300.010.0495922
AATCGAA54500.09.6586185
CTCGTTT156250.09.4072751
CGCATAT28050.09.4034951
GTCAGTA202750.09.2512151
CGGCTAT54850.09.1656761
ATCATGC228000.09.1560627
CGCTAGT16300.09.1283571
GCGGCTT191400.09.08582115
GTCGTTT107650.09.0260871