FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l03n01 eb-nil-24hr dox.34100000007e33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l03n01 eb-nil-24hr dox.34100000007e33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47224284
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACT123900.011.7731331
AATCCGA77050.011.53601424
CGTGAAA36400.011.37391633
TCGAATG66150.010.8822912
CGCATAT18350.010.1976731
CTCGAAT132050.010.1245411
ACGTGAA48600.010.09386432
TGAAACG41750.010.07798335
AAACGAT43400.09.95241137
ATCCGAA89750.09.88035725
AAATCCG87300.09.715610523
GTATCAT92900.09.44385055
TATCATG93250.09.263666
CGGCTAT35750.09.2073761
TCGTTTT104750.09.0841972
AACGATC46650.09.01774638
TTACGGC27200.08.84958540
GTCACGT47950.08.82527929
GAAACGA47850.08.79283436
TGTAGCT221950.08.7469437