FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l03n01 eb-nil-0hr.34100000007dc7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l03n01 eb-nil-0hr.34100000007dc7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32015087
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACT93200.010.5460161
CTCGAAT68000.09.8792781
CCGGCAT41950.09.1355521
TATCTCG25200.08.92706739
TCGAATG36950.08.7668822
CAATCGA13450.08.5299214
TGTAGCT165400.08.5139927
CTCAGAT96250.08.4317871
CGAATCG9400.08.3760133
ACTGTAG139850.08.3644095
CGGGAAT29800.08.245711
CTCAAAT127500.08.0802331
GTCGAAT37300.07.85692021
CGGCTAT19100.07.7898381
CGATTAG26050.07.7719844
TTACGGC17100.07.63029340
CGCTAAT6804.7293724E-107.62494561
CACTGTA162550.07.5838674
TCGTTTT68950.07.5365892
TAGCTGT170750.07.53613579
Produced by FastQC (version 0.11.2)