FastQCFastQC Report
Tue 14 Apr 2015
C55MVACXX l02n01 ap_n1.34100000007dba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l02n01 ap_n1.34100000007dba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25305695
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA42650.025.1819231
TCGTATG44500.023.61075645
ATCGGAA47650.022.6129822
ACACGTC48650.022.24082413
AATCTCG48300.021.79730641
CTCGTAT50100.020.9716344
ACGTCTG53200.020.63565815
CGTCTGA55950.019.5007716
CACGTCT63300.017.48482114
TCTCGTA62850.016.89602943
CACACGT64500.016.84519812
GCACACG75300.014.84738811
CGGAAGA79250.014.0504814
ATCTCGT77250.013.95012242
GAATCTC84700.012.74946640
ACATGTC87000.012.33607732
TCGGAAG95400.011.7662323
CACATGT101700.010.82167731
TCACATG104100.010.74582730
TCTGAAC105150.010.50477218
Produced by FastQC (version 0.11.2)