FastQCFastQC Report
Tue 14 Apr 2015
C55MVACXX l02n01 ap_eb1.34100000007d34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l02n01 ap_eb1.34100000007d34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22369716
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGA31500.012.50251524
AAATCCG33350.011.6041223
GTCGGTT31550.011.0869981
GGGGTAT37750.010.8801791
ATCCGAA37450.010.57776425
GTCCGCT34150.09.9124771
TATCATG39750.09.9034686
GGGGGTT77150.09.8576981
GTCCTAT25850.09.7777541
CAATTGA25000.09.6278694
ACGTATA11700.09.4215618
GTCCCGT22800.09.3041051
GTATCAT43000.09.2072635
GTCGCTT35400.09.0524621
ATTACGG13600.08.93268539
GTCCAAT39100.08.8307231
GTCCGTT26950.08.7924961
CGCTAGT4458.066854E-88.6212561
GCCGTAT16700.08.5134521
GTCCATT65200.08.48008251
Produced by FastQC (version 0.11.2)