FastQCFastQC Report
Tue 14 Apr 2015
C55MVACXX l02n01 alp_n1.34100000007dfe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l02n01 alp_n1.34100000007dfe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28216386
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG23450.024.4665332
CGTCCGC33900.017.4530733
GATCGGA34250.017.1966821
TCGTATG38050.015.84597645
ACACGTC39400.015.18699813
ACGTCTG41700.014.51174715
ATCGGAA40750.014.2973772
CTCGTAT42500.013.92213144
GTCCGCA43000.013.60342834
GTCACGT44400.013.38341429
CGTCTGA46550.013.04810816
CGCACAT45550.013.03891137
AGTCACG47100.012.80711828
CACGTCT53650.011.4049514
TCTCGTA54250.011.1555843
CACACGT55850.011.07633412
TCACGTC55850.011.0023530
ATCCGAA38850.010.71837625
AATCCGA41850.010.43196224
AAATCCG41300.010.132997523
Produced by FastQC (version 0.11.2)