FastQCFastQC Report
Tue 14 Apr 2015
C55MVACXX l02n01 alp_eb1.34100000007d5e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l02n01 alp_eb1.34100000007d5e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25477747
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA38900.021.5113432
GATCGGA40750.020.598181
ACACGTC41550.020.51850913
TCGTATG42950.019.85338843
AATCTCG44000.019.2765939
CGTATGC44450.018.93048944
ACGTCTG47050.018.79021815
CGTCTGA48050.018.07144416
TCTCGTA53000.015.91677941
CACGTCT56550.015.59335714
CTCGTAT55950.015.36081242
CACACGT58200.015.035027512
GTATGCC56100.014.99929145
GTAATCT59200.014.32699937
TAATCTC63900.013.20279838
GCACACG75450.012.13424511
CGGAAGA75500.011.9175574
ATCTCGT75850.011.27120740
AATCCGA31800.011.04024924
TCGGAAG84150.010.7727133
Produced by FastQC (version 0.11.2)