FastQCFastQC Report
Tue 14 Apr 2015
C55MVACXX l02n01 a_n1.34100000007d77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l02n01 a_n1.34100000007d77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21841533
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGATC22050.019.99739338
GATCGGA23150.019.3007181
AAACGAT24350.018.10843837
TGAAACG25550.017.69904935
CGTGAAA25200.017.58650833
GAAACGA26800.016.87314636
ACGTGAA27800.016.83407232
TCGTATG28250.016.16673745
ACACGTC30050.015.34594113
GTCACGT30250.015.32789629
ACGTCTG32100.014.57683715
ATCGGAA31050.014.4894372
CTCGTAT31450.014.37871844
AGTCACG33750.013.67155128
CGTCTGA34500.013.36717916
CACGTGA35800.013.20258831
ACGATCT38750.012.8887339
TCACGTG36000.012.81716730
CACGTCT38850.011.75447114
TCTCGTA39350.011.60630943
Produced by FastQC (version 0.11.2)