FastQCFastQC Report
Tue 14 Apr 2015
C55MVACXX l02n01 a_eb1.34100000007cff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l02n01 a_eb1.34100000007cff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21335199
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA26800.015.0726921
AATCCGA32000.014.55722424
TCGTATG27800.014.48623343
ACACGTC28950.014.21970813
ATCGGAA27900.014.1903672
ACGTCTG30500.013.128695515
AAATCCG35200.012.9116723
ATCCGAA36050.012.67436925
CGTATGC32100.012.47582744
CGTCTGA33250.011.83989416
TATCATG42300.011.061356
AGTGATC37250.010.81122836
ATTACGG15150.010.54357339
CTCGTAT38400.010.48733142
GTATCAT44000.010.2761055
TCTCGTA38300.010.16124141
CACACGT41650.010.09984812
CACGTCT40600.010.02884514
GTATGCC41800.09.7421945
TCCGAAG49450.09.740540526
Produced by FastQC (version 0.11.2)