FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l01n01 se394.3410000000814a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l01n01 se394.3410000000814a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19110531
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC290260.15188484296956478TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC49450.029.30046744
GCACACG53600.028.41384311
GTATGCC52300.027.78975145
ACACGTC54750.027.77593413
CTCGTAT56250.026.1177342
ACGTCTG58050.025.96442415
CACACGT59150.025.74779312
CACGTCT58800.025.70976314
TCGTATG56950.025.6785943
CATCACG57150.025.1933433
CACGATC56400.024.9310836
ACGATCT58900.023.91095737
CGATCTC59300.023.82598138
CAGTCAC61250.023.81235527
GATCTCG60900.023.6063839
CCAGTCA64350.023.18993626
CGTCTGA67200.022.56299416
ACTCCAG67000.022.36600923
AGCACAC70500.021.63437710
GAACTCC70350.021.61853221
Produced by FastQC (version 0.11.2)