FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l01n01 se393.34100000008120.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l01n01 se393.34100000008120.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12356565
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCC3306592.6759783159802097TruSeq Adapter, Index 21 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC405850.042.5302813
GCACACG407800.042.38207611
CACACGT407100.042.3333812
ACGTCTG407050.042.28884515
AGTCACG402950.042.21707528
GTATGCC398000.042.2049445
CACGTCT409450.042.1233814
CGTATGC399400.042.02320544
GTCACGT403500.041.82889629
CTCGTAT402050.041.73927342
CGTCTGA413150.041.56101216
TCGTATG405400.041.50568843
CGATCTC399250.041.40120338
GATCTCG403600.041.3005939
CCAGTCA411500.041.29218326
CAGTCAC411700.041.26118527
CGTTTCG406300.041.23908633
AGCACAC421250.041.15703210
GAACTCC413350.041.1436721
ACTCCAG414300.041.1028623
Produced by FastQC (version 0.11.2)