FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l01n01 se392.34100000008106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l01n01 se392.34100000008106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8971302
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG2559552.8530418438706TruSeq Adapter, Index 14 (97% over 44bp)
CCTAAGTGACTTCGTTTCAGACAAACCTCCACAATGTTACGCGCAATTTAA200510.2235015608659702No Hit
GCTCTCAACTCAGCGATTTGGCTAATCACCAATGCCTTCTAACCATTTCCA142870.1592522467753287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT314700.042.9979312
CACGTCT314450.042.8890314
ACGTCTG315800.042.73418415
TCGTATG305800.042.6420745
GTCACAG311550.042.6234329
CGTATCT306450.042.6094139
ACACGTC317600.042.5840713
CGTCTGA316600.042.52672216
AGTCACA312750.042.51911528
CAGTCAC313400.042.4823927
AGCACAC320350.042.3940710
CTCGTAT307450.042.30345544
TCCGTAT310600.042.24920737
GTATCTC309950.042.22987440
ACTCCAG315800.042.1943723
CCGTATC309500.042.167738
GTTCCGT312500.042.0213635
CCAGTCA316700.042.0041926
GCACACG323450.041.95298411
GATCGGA315000.041.927341