FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l01n01 se391.341000000080eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l01n01 se391.341000000080eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10896785
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC2958092.714644732368309TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT352150.042.8107742
AGTCACC353750.042.75103828
GTATGCC354450.042.73209445
TCGTATG354600.042.6932243
GTAATCT353300.042.51183337
CAGTCAC358300.042.33475527
TGTAATC354850.042.32516536
CGTATGC357950.042.28283744
CCAGTCA359250.042.1664226
CCTTGTA357700.042.1190533
TCTCGTA358250.042.0941941
ACTCCAG360500.042.08727323
CTTGTAA357200.042.0776234
GTCACCT359300.042.03371429
TAATCTC357550.042.01300438
CACGTCT365350.041.93735514
CACACGT365900.041.91735512
CGTCTGA364200.041.9091816
ACGTCTG365700.041.88491415
CTCCAGT361450.041.88397224