FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l01n01 se390.341000000080c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l01n01 se390.341000000080c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7993891
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG14800.020.9863628
ACGGCTA15500.019.88703732
CACGTCT18350.019.002314
TCGTATG17250.018.91144843
CGTCTGA19100.018.72726416
CTCGTAT17300.018.46653442
ACACGTC19300.018.30007613
CAGTCAC17250.018.26732827
ACGTCTG19300.018.18351415
GTATGCC17350.018.15465545
CGTATGC17550.018.07596244
CACACGT20050.017.83993512
GCTACAT17100.016.84015735
GTCACGG18600.016.8194129
GGCTACA17050.016.6255434
GCACACG22600.016.32472811
ACTCCAG21950.015.88912923
CACGGCT19350.015.58534531
TCTCGTA22750.014.63603741
CGGCTAC20300.014.51784433