FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l01n01 se389.341000000080a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l01n01 se389.341000000080a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11200258
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC375510.33526906255195194TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGC54900.035.5843531
ACTAGCT56050.034.88350332
GTATGCC58800.034.6658345
CGTATGC58650.034.63940444
CTCGTAT59000.034.24233642
TCGTATG60050.033.86853843
TAGCTTA58700.033.15167234
TCACTAG59400.032.7759230
CTAGCTT62000.031.74972533
TCTCGTA66650.030.7846241
GCTTATC63800.030.60808836
CAGTCAC66100.030.40847427
CTTATCT65050.030.29619637
CCAGTCA67400.030.05626
AGCTTAT68050.028.96097235
TCCAGTC71000.028.72224625
ACTCCAG70900.028.7212623
TATCTCG69950.028.68905439
CTCCAGT72200.028.11451324
CGTCTGA73950.028.01713416
Produced by FastQC (version 0.11.2)