FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l01n01 se388.3410000000808e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l01n01 se388.3410000000808e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12826427
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC3871493.018369807897398TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT447050.042.92745242
AGTCACG452450.042.75351728
TCGTATG451900.042.72130643
GTATGCC451850.042.67740645
CGTATGC453350.042.52610844
TCTCGTA451000.042.5067541
GTCACGA458500.041.948529
CAGTCAC464450.041.62563727
CCAGTCA465150.041.55364226
TCACGAT465300.041.4176930
ATCTCGT464000.041.3062940
AGATCTC464350.041.2509338
CAGATCT461700.041.20946537
TCCAGTC473000.040.92111625
CTCCAGT473600.040.8440224
CGTCTGA479150.040.73783516
GATCAGA469300.040.63766534
AACTCCA478300.040.48763722
ACGATCA473800.040.45840532
ACGTCTG483850.040.4583515
Produced by FastQC (version 0.11.2)