FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l01n01 se387.34100000008064.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l01n01 se387.34100000008064.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11973503
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC435880.36403715771399564TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT61650.035.94642342
TCGTATG65500.034.04034443
CGTATGC66000.033.7833144
GTCACAC68250.032.77997229
GTATGCC68250.032.66916345
AGTCACA70800.031.88571728
TCTCGTA70050.031.82863841
ACACTTG71250.031.419832
CAGTCAC73050.030.8430227
CACGTCT76600.030.19033414
TCACACT74500.029.99976530
CACTTGA74950.029.95577833
CACACGT77600.029.71431412
ACACGTC78250.029.55373413
ACGTCTG78750.029.45178815
CGTCTGA78900.029.25323516
CCAGTCA77050.029.18340126
ACTCCAG80000.028.68791823
CTCCAGT79750.028.58464824
CACACTT78650.028.44376831
Produced by FastQC (version 0.11.2)