FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l01n01 se386.3410000000804b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l01n01 se386.3410000000804b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18646407
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1142860.6129116456591343TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT148400.039.41691642
TCGTATG154100.038.03233343
GTATGCC155700.037.61341545
CGTATGC157550.037.14338344
TCTCGTA160100.036.71913541
AGTCACC162050.036.1677528
CAGTCAC166600.035.39727827
CCAGTCA171800.034.29995726
CTCCAGT180350.032.79304524
ACTCCAG182150.032.63635323
ATCTCGT182950.032.36665740
TCCAGTC183100.032.3429525
GAACTCC187000.031.99153521
AGATCAT190900.030.92380535
TCATCTC192600.030.67495738
AACTCCA194450.030.60475322
TGAACTC196600.030.47213720
GTCACCA194250.030.32244129
CGTCTGA198100.030.08177216
CATCTCG196800.029.974539
Produced by FastQC (version 0.11.2)