FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l01n01 se385.34100000008021.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l01n01 se385.34100000008021.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20627992
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1502370.7283161637836586TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT193400.040.11046242
TCGTATG196200.039.6759343
AGTCACG200200.038.7865328
TCTCGTA203650.038.14669441
CGTATGC206500.037.7519644
GTATGCC207900.037.51938245
ATATCTC207300.037.4752238
CAGTCAC211100.036.92366827
TATCTCG214350.036.2740539
CCAGTCA214650.036.26066626
CCAATAT213300.036.12476335
GCCAATA214850.035.7172934
CTCCAGT219700.035.32923524
ACTCCAG223600.034.90890523
AATATCT223400.034.8042437
AACTCCA225650.034.5292522
TCCAGTC230250.034.0385325
TCTGAAC231300.033.98232318
GAACTCC234650.033.3094721
ATCTCGT235800.033.17467540
Produced by FastQC (version 0.11.2)