FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l01n01 se384.34100000008007.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l01n01 se384.34100000008007.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18386343
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC2180501.1859345819883813TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT267300.041.1162442
TCGTATG269100.041.04242743
GTCACAC270550.040.2734229
GTATGCC274950.040.1127845
ACACAGT271700.040.08779532
AGTCACA273850.039.93626428
CGTATGC278350.039.67130744
TCTCGTA278850.039.3969641
CAGTCAC278100.039.23830827
CACACAG280000.039.07272331
CACAGTG281500.038.74403833
ACAGTGA280750.038.60819634
CCAGTCA282150.038.54754626
TCACACA283750.038.50302530
AGTGATC283100.038.3040236
ACTCCAG286850.038.1300223
CTCCAGT288400.037.76782224
TCCAGTC292500.037.22972525
CAGTGAT292550.037.07430635
GAACTCC298400.036.80978821
Produced by FastQC (version 0.11.2)