FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l01n01 se383.34100000007fef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l01n01 se383.34100000007fef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19204008
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC4090042.1297845741368158TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT490650.042.22108542
TCGTATG492450.042.19054443
GTATGCC496550.041.73916645
CGTATGC497550.041.66431844
TCTCGTA502700.041.28957441
CAGTCAC509600.041.19656827
CCAGTCA511450.040.91563426
CCAATCT507000.040.7572437
GACCAAT507100.040.739835
GTCACTG516300.040.73863229
CTCCAGT519700.040.3014524
AATCTCG519050.040.30113639
ACCAATC512250.040.27322836
CACTGAC521500.040.2189531
ACTGACC518400.040.14246732
TCACTGA523900.040.07461530
AGTCACT526650.039.95542528
ACTCCAG526850.039.92704823
TCCAGTC525750.039.8712625
CAATCTC523050.039.6832538
Produced by FastQC (version 0.11.2)