FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l01n01 se382.34100000007fc5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l01n01 se382.34100000007fc5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19257180
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC1166120.6055507608071379TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG135200.041.67086832
CACTTAG135900.041.4864731
CTCGTAT144850.039.10883742
TCGTATG145700.039.0508443
TTAGGCA145250.038.48930734
TCACTTA151000.037.4439130
CGTATGC152100.037.4374544
TCTCGTA154100.036.76119641
GTATGCC155600.036.68200745
TAGGCAT158050.035.55721735
CAGTCAC162200.035.20704327
GTCACTT162450.034.97100429
CCAGTCA169350.033.7340626
AGTCACT169550.033.58701328
CTCCAGT173150.033.0138924
AGGCATC173500.032.31308436
GCATCTC175000.032.2295338
ATCTCGT178450.031.97204240
GGCATCT176100.031.92575837
ACTCCAG180250.031.9193523
Produced by FastQC (version 0.11.2)