FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l01n01 se381.34100000007fac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l01n01 se381.34100000007fac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16967460
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1643570.9686600115750973TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT200000.040.9347842
TCGTATG204900.040.25303343
GTATCTC205750.040.12988738
TGTATCT207350.039.84110637
ATGTATC208100.039.59998736
GATGTAT209650.039.4572135
TCTCGTA209450.039.19529341
AGTCACC213100.039.0370628
CGTATGC211300.038.90685344
CAGTCAC215050.038.7273527
GTATGCC213600.038.51928745
TATCTCG218200.037.8814139
CCAGTCA220400.037.7771826
CTCCAGT225100.036.91122424
ACTCCAG227350.036.6489823
CGATGTA225350.036.62827734
AACTCCA231300.036.02045422
TCCAGTC233350.035.8350325
TCTGAAC236700.035.4117918
ATCTCGT235050.035.16580640
Produced by FastQC (version 0.11.2)